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It is accomplished by a limited number of enzymes with broad substrate specificities. Reactions catalyzed by xenobiotic-biotransforming enzymes are divided into two groups, called phase I and phase II, leading to an increase in hydrophilicity of xenobiotic, greatly enhancing their elimination.

Among the phase I biotransforming enzymes, cytochromes P450 (CYP450) rank first in terms of oxidation catalytic versatility and the broad number of xenobiotics they detoxify or activate to reactive intermediates, that may or may not be taken in charge by phase II enzymes.

Being the major elimination route for many drugs, CYP450 enzymes play a very drug effects more oxycontin side important role in the detoxication of xenobiotics, but may also lead to toxic or tumorigenic metabolites. See also our chapter "Biochemicals & CYP450s" for further information about CYP450 microsomes recombinant enzymes, or our list of analytical standards, substrates and metabolites on page V.2. >Specificity: no cross reaction with other CYP are observed or known >Application media: microsomes, hepatocytes, tissue extracts, cell lines, cell membrane fractions >Preanalytical phase: see booklet >Stability: 6 months > Sample amount: 50 |jg of total protein >Storage: -80°C > Shipping: dry ice >Size: 24 dtn X""Ji Calibration curve obtained for peptide related to CYP2C8 Chromatograms obtained for CYP2C8 Find below a current list of assay kits.

Do not hesitate to contact us for an updated list: CYP1A2 (human) Assay Kit T05007 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% : Monkey: 0% 0.75 fmol/jg of total protein 0.75 to 150 fmol/jg of total protein 0% CYP2A6 (human) Assay Kit T05008 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 0% 0.75 fmol/jg of total protein 0.75 to 150 fmol/jg of total protein 0% CYP2B6 (human) Assay Kit T05009 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 0% 0.75 fmol/jg of total protein 0.75 to 150 fmol/jg of total protein 0% CYP2C8 (human) Assay Kit T05010 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 0% 0.75 fmol/jg of total protein 0.75 to 150 fmol/jg of total protein 0% CYP2C9 (human) Assay Kit T05011 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 0% 0.75 fmol/jg of total protein 0.75 to 150 fmol/jg of total protein 0% CYP2C19 (human) Assay Kit T05012 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 0% 0.75 fmol/jg of total protein 0.75 to 150 fmol/jg of total protein 0% CYP2D6 (human) Assay Kit T05013 >Specificity: >Human: 100% : Monkey: 0% >Mouse: 0 % >Limit of quantification: 0.75 fmol/jg of total protein >Standard curve range: 0.75 to 150 fmol/jg of total protein CYP3A4 (human) Assay Kit >Specificity: : Human: : Mouse: >Limit of quantification: >Standard curve range: 0% 100% > Monkey: 0% 0.75 fmol/jg of prescription generic xanax total protein 0.75 to 150 fmol/jg of total protein ► Human Enzyme UDP GlucuronosylTransferase (UGT) Assays UGTs are a family of enzymes not only capable of the detoxification and activation of drugs and xenobiotics, but are also involved in the metabolism of important endogenous substrates, e.g. The biochemical characterization of the different isoforms is complicated due to the broad substrate specificity (and overlapping substrate affinities (i.e. one substrate is metabolized by more than one isoform). Glucuronidated compounds are susceptible to deconjugation by beta-glucuronidase, an enzyme that is widely distributed in mammalian tissues and localized intracellularly in lysosomes and endoplasmic reticulum. Combined actions of UGTs and beta-glucuronidase may play a role in futile cycling in which conjugated metabolites undergo successive cycles of synthesis to the glucuronide and hydrolysis back to the aglycone. >Specificity: human UGT listed below >Application media: microsomes, tissue extracts, cell lines, cell membrane fractions prescription generic xanax >Preanalytical phase: see booklet >Stability: 6 months : Sample amount: 50 jg of total protein >Storage: -80°C : Shipping: dry ice >Size: 24 dtn Find below a current list of assay kits. Do not hesitate to contact us for an updated list: UGT1A1 (human) Assay Kit T05015 > Limit of quantification: > prescription generic xanax Standard curve range: at least 3 fmol/jg of total protein 3 to 300 fmol/jg of total protein UGT1A3 (human) Assay Kit T05016 > Limit of quantification: > Standard curve range: at least 3 fmol/jg of total protein 3 to 300 fmol/jg of total protein UGT1A6 (human) Assay Kit T05017 > Limit of quantification: > Standard curve range: at least 3 fmol/jg of total protein 3 to 300 fmol/jg of total protein UGT1A9 (human) Assay Kit T05018 > Limit of quantification: > Standard curve range: at least 3 fmol/jg of total protein 3 to 300 fmol/jg of total protein UGT2B7=UGT2B9 (human) Assay Kit T05019 >Limit of quantification: at least 3 fmol/jg of total protein >Standard curve range: 3 to 300 fmol/jg of total protein UGT2B15=UGT2B8 (human) Assay Kit T05020 : Limit of quantification: at least 3 fmol/|jg of total protein : Standard curve range: 3 to 300 fmol/jg of total protein Multiplex Protein Assay Kits : Stability: : Storage: : Shipping: : Size: 6 months Sample amount: 50 jg of total protein -80°C > Limit of quantification: see each single protein dry ice Standard curve range: see each single protein 24 dtn DMPK A (MDR1, BCRP) T05021 : Specificity: : Application media: : Preanalytical phase: refer to each single protein (MDR1, BCRP) prescription generic xanax microsomes, hepatocytes, tissue extracts, cell membrane fractions see booklet DMPK B (MDR1, BCRP, OATP1B1, OATP1B3) T05022 : Specificity: : Application media: : Preanalytical phase: refer to each single protein (MDR1, BCRP, OATP1B1 and OATP1B3) microsomes, hepatocytes, tissue extracts, cell membrane fractions see booklet DMPK C (MDR1, OAT1, OAT3, OCT2) T05023 : Specificity: : Application media: : Preanalytical phase: refer to each single protein (MDR1, OAT1, OAT3 and OCT2) microsomes, hepatocytes, tissue extracts, cell membrane fractions see booklet DMPK D (CYP1A2, CYP2B6, CYP2C9, CYP3A4) T05024 : Specificity: : Application media: : Preanalytical phase: human, CYP450 1A2, 2B6, 2C9, 3A4 microsomes, hepatocytes, tissue extracts, cell membrane fractions see booklet Introduction to AChE(r) technology & prescription generic xanax formats used Before presenting our range of enzyme immunoassays, we want to give you some very useful information on the AChE(r) technology and the various formats of assays used.



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