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Therefore, Bertin Pharma is offering a first line of MS2Plex(r) kits for membrane protein quantification focused on drug metabolism. We can design kits or offer our technological platform for any other download oxycontin by lil whyte proteins, biomarkers and biologics. Introduction to membrane protein quantification by LC-MS/MS ► Sample process workflow After collecting your sample, membrane should be isolated and proteins solubilised.

The proteins are then alkylated, precipitated and digested with trypsin (enzymes are provided in the MS2Plex(r)kits).

These classical steps are checked using a patented Monitoring Protein to check the purification and enzymatic digestion steps. Prepare tryptic peptide library Reduction, Alkylation and Tryptic digestion Samples (Tissues, cells) Protein samples ^Tryptic peptide library The peptides generated are mixed with Internal Standards (IS, peptides labelled with 13C or 15N stable isotopes (at a known and precise concentration (identical to the one included into the calibration standards) before injection in the LC-MS/MS system. The set of standards peptides is also provided to allow the construction of a standard curve, as well as a set of Quality Controls at High (0.8 x ULOQ), Medium (0.5 x ULOQ(14)) and Low (2 x LLOQ(15)) concentration levels. A Test System Sample provided download oxycontin by lil whyte for injection will permit to verify that the equipment is performing as expected before submitting the analytical batch to the precious samples. ► MS2Plex(r) benefits 0 A unique & large range for LC-MS/MS membrane protein quantification 0 Highly specific peptides & Internal Standards 0 High sensitivity (fmol/jg of total protein) & specificity by mass filter 0Simultaneous quantification: up to 34 proteins in a single run 0 State-of-the art validated assays 0 Numerous peer review scientific publications 0 Enzymes included Further to the kits already available, more than 300 proteins (from human, mouse, rat or monkey) have been measured using this technology.

If you do not find the kit you need, feel free to contact us. ► ABC Transporters Assays ABC (ATP binding cassette) transporters comprise one of the largest of all paralogous protein families. They are found in all species, play a wide variety of physiological roles and are of very considerable medical and economic importance. In microorganisms, ABC transporters are central to antibiotic and antifungal resistance, while in human many are associated with genetic diseases and cancer drug resistance. The name ABC (ATP Binding Cassette) transporter was coined in 1990, cementing a general recognition of the importance of this evolutionarily related but functionally diverse family of proteins. >Application media: >Preanalytical phase: >Stability: >Storage: >Size: 6 months -80°C 24 dtn >Sample amount: >Shipping: microsomes, hepatocytes, tissue extracts, cell lines, cell membrane fractions see booklet 50 |jg of total protein dry ice • n> Calibration curve obtained for peptide related to MDR1 MDR1 chromatograms T05000 Find below a current list of assay kits. Do not hesitate to contact us for an updated list: MDR1 (human/monkey) Assay Kit >Specificity: : Human: : Mouse: >Limit of quantification: >Standard curve range: 100 % 100% : Monkey: 0% 0.15 fmol/jg of total protein 0.15 to 30 fmol/jg of total protein BCRP (human/mouse/monkey) Assay Kit T05001 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 100 % 0.75 fmol/|jg of total protein 0.75 to 150 fmol/jg of total protein 100 % ► SLC Transporters Assays SoLute Carrier protein (SLC) is a wide family (51) of transporters facilitating small (endogenous and xenobiotics) molecule transport through plasma and other cellular membrane by diffusion or by co-transport of endogenous (organic) ions to provide the driving force. Certain SLC transporters exhibit efflux properties or download oxycontin by lil whyte are bidirectional, which depends on the concentration gradients of substrate and coupled ions across the membrane. Among SLC super-family, organic anion transporting polypeptides (OATPs), organic anion transporters (OATs) and organic cation transporters (OCTs) subfamilies are functionally well characterized and studied for their implication in physiology, pathophysiology, and pharmacology. OAT1 (human/mouse/monkey) Assay Kit T05005 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 100 % 0.3 fmol/jg of total protein 0.3 to 30 fmol/jg of total protein 100 % OAT3 (human) Assay Kit T05006 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 0% 0.3 fmol/jg of total protein 0.3 to 30 fmol/jg of total protein 0% OATP1B1 (human) Assay Kit T05002 > Specificity: > Human: > Mouse: > Limit of quantification: > Standard curve range: 100% > Monkey: 0% 0.15 fmol/jg download oxycontin by lil whyte of total protein 0.15 to 30 fmol/jg of total protein 0% OATP1B3 (human/monkey) Assay Kit T05003 >Specificity: >Human: 100% : Monkey: 100% >Mouse: 0 % >Limit of quantification: 2.25 fmol/jg of total protein >Standard curve range: 2.25 to 30 fmol/jg of total protein OCT2 (human/monkey) Assay Kit hydrocodone cough supressant >Specificity: >Human: 100% : Monkey: 100% >Mouse: 0 % fibromyalgia oxycodone >Limit of quantification: 0.3 fmol/jg of total protein >Standard curve range: 0.3 to 30 fmol/jg of total protein ► Human CYP450s Assays Xenobiotic biotransformation is the principal mechanism for maintaining homeostasis during organism exposure to foreign molecules such as drugs.



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